ONKOSIGHT ADVANCED^TM NEXT GENERATION SEQUENCING

Empowering providers with advanced genetic sequencing to improve cancer patient management.

OnkoSight Advanced^TM is a next-generation sequencing (NGS) assay that enables cutting edge DNA mutational profiling of tumor samples. OnkoSight Advanced^TMfocuses on only actionable mutations in common cancers. This targeted approach helps personalize patient treatment and management while greatly reducing the number of unclear variants that often occur in the results of broader NGS panels.

Genes included on OnkoSight Advanced^TMNGS panels are those with clear therapeutic, diagnostic, or prognostic value and those for which there are late-stage clinical trials available. National guidelines-recommended genes are included on each tumor-specific panel.

Critical genomic biomarkers relevant to immunotherapy; Tumor Mutation Burden (TMB) and Microsatellite Instability (MSI) are also reported with every OnkoSight Advanced^TM analysis.

Entire exonic coding regions and selected introns are broadly covered among genes targeted for analysis. Coverage of whole exonic coding regions represents an improvement over targeted hotspot approaches, and minimizes the risk of potential false negatives due to lack of coverage across clinically relevant genomic loci.

Solid Tumor

GENERAL SOLID TUMOR PANEL (50 genes, TMB, and MSI)

Test Code TJ16-5

AKT1	APC	ARID1A	ATM	BRAF	BRCA1	BRCA2	CDH1	CDKN2A
CTNNB1	EGFR	EPCAM	ERBB2	ERBB4	FBXW7	FGFR1	FGFR2	FGFR3
GNA11	GNAQ	GNAS	HRAS	IDH1	IDH2	KDR	KIT	KRAS
MEN1	MET	MLH1	MSH2	MSH6	NOTCH1	NRAS	PDGFRA	PIK3CA
PMS2	POLE	PTEN	PTPN11	RB1	RET	SMAD4	SMO	STK11
TERT	TSC1	TSC2	TP53	VHL

COLORECTAL CANCER PANEL (32 genes, TMB, and MSI)

Test Code: TH50-8

AKT1	APC	ATR	BRAF	CHEK1	EGFR	EPCAM	ERBB2	ERBB3
ERBB4	FGFR1	FGFR2	FGFR3	FGFR4	HRAS	KRAS	MET	MAP2K1
MLH1	MSH2	MSH6	NRAS	NTRK1	PIK3CA	PMS2	POLD1	POLE
PTEN	SMAD4	STK11	TERT	TP53

CENTRAL NERVOUS SYSTEM TUMOR PANEL (29 genes, TMB, and MSI)

Test Code: TH57-3

APC	ATR	ATRX	BRAF	CDKN2A	CHEK1	CTNNB1	EGFR	ERBB2
H3F3A	H3F3B	H3F3C	HIST1H3B	HIST1H3C	IDH1	IDH2	MYC	MYCN
NF1	NF2	NTRK1	PDGFRA	PIK3CA	PTEN	SMARCA4	SMARCB1	TERT
TP53	VHL

GASTROINTESTINAL STROMAL TUMOR PANEL (10 genes, TMB, and MSI)

Test Code: TH58-1

BRAF	ERBB3	KIT	NF1	NTRK1	PDGFRA	SDHA	SDHB	SDHC
SDHD

GYNECOLOGIC NEOPLASMS PANEL (50 genes, TMB, and MSI)

Test Code: TH53-2

AKT1	AR	ATM	BRAF	BRCA1	BRCA2	BRIP1	CDH1	CDK12
CHEK1	CHEK2	EGFR	EPCAM	ERBB2	ERBB3	ESR1	FANCA	FANCC
FANCL	FGFR1	FGFR2	FOXA1	KRAS	MLH1	MRE11A	MSH2	MSH6
MUTYH	NBN	MYC	NF1	NTRK1	PALB2	PIK3CA	PMS2	POLE
PTEN	RAD50	RAD51B	RAD51C	RAD51D	RAD54L	RB1	RECQL4	SLX4
SMARCA4	TBX3	TERT	TP53	XRCC2

MELANOMA PANEL (26 genes, TMB, and MSI)

Test Code: TH59-9

BAP1	BRAF	BRCA2	CDK4	CDKN2A	CTNNB1	EIF1AX	GNA11	GNAQ
GNAS	KIT	MAP2K1	MITF	NBN	NF1	NRAS	NTRK1	PDGFRA
PIK3CA	PTEN	RB1	SF3B1	SMO	SRC	TERT	TP53

LUNG CANCER PANEL (22 genes, TMB, and MSI)

Test Code: TH49-0

AKT1	ALK	ATR	BRAF	CHEK1	DDR2	EGFR	ERBB2	ERBB3
FGFR1	KRAS	MAP2K1	MET	NRAS	NTRK1	PIK3CA	POLD1	POLE
ROS1	STK11	TERT	TP53

PANCREATICOBILIARY TUMOR PANEL (22 genes, TMB, and MSI)

Test Code: TK84-0

ARID1A	BRAF	BRCA1	BRCA2	ERBB2	FGFR1	FGFR2	FGFR3	IDH1
IDH2	KRAS	MLH1	MSH2	MSH6	NRAS	PALB2	PIK3C2G	PMS2
STK11	TGFBR2	TP53	VHL

PROSTATE CANCER PANEL (34 genes, TMB, and MSI)

Test Code: TH48-2

AR	ATM	ATR	BARD1	BRCA1	BRCA2	BRIP1	CDK12	CHEK1
CHEK2	EPCAM	FAM175A	FANCA	FANCL	GEN1	HOXB13	MLH1	MRE11A
MSH2	MSH6	MUTYH	NBN	NTRK1	PALB2	PMS2	PPP2R2A	PTEN
RAD51B	RAD51C	RAD51D	RAD54L	RB1	TERT	TP53

Chronic Lymphoid Neoplasm

Chronic Lymphoid Neoplasm Panel (41 genes, TMB, and MSI)

Test Code: TH55-7 (for BM and PB specimen); TJ93-4 (for FFPE specimen)

ATM	BCL2	BCL6	BIRC3	BRAF	BTK	CARD11	CD79B	CDKN2A
CREBBP	CXCR4	DNMT3A	EP300	EZH2	IDH1	IDH2	IKZF1	IRF4
JAK1	JAK3	KMT2D	KRAS	MAP2K1	MEF2B	MYC	MYD88	NOTCH1
NOTCH2	NRAS	PIK3CA	PIK3CG	PLCG2	PTPRD	RHOA	SETD2	SF3B1
STAT3	STAT5B	TET2	TNFRSF14	TP53

Multiple Myeloma

Multiple Myeloma Panel (50 genes, TMB, and MSI)

Test Code: TL67-3 (for BM and PB specimen); TL68-1 (for FFPE specimen)

AKT1	AKT2	AKT3	ATM	ATR	B2M	BIRC3	BRAF	CCND1
CDK4	CDKN1B	CDKN2A	CXCR4	CYLD	DIS3	DNMT3A	EGFR	FAM46C
FGFR3	FUBP1	IDH1	IDH2	IGF1R	IKZF1	IRF4	JAK2	KDM6A
KRAS	MAX	MYC	MYD88	NF1	NFKBIA	NOTCH1	NRAS	PIK3CA
PIK3CG	PIK3R1	PIK3R2	PIM1	PRDM1	PSMB8	PTPN11	RB1	STAT3
TERT	TET2	TGFBR2	TP53	TRAF2

Myeloid Malignancies

General Myeloid Panel (50 genes)

Test Code: TL95-4

ABL1	ANKRD26	ASXL1	ATRX	BCOR	BCORL1	BRAF	CALR	CBL
CCND2	CDKN2A	CEBPA	CSF3R	CUX1	DDX41	DNMT3A	ETNK1	ETV6
EZH2	FBXW7	FLT3	GATA2	HRAS	IDH1	IDH2	JAK2	KDM6A
KIT	KMT2A	KRAS	MAP2K1	MPL	MYD88	NF1	NPM1	NRAS
PDGFRA	PHF6	PTEN	PTPN11	RUNX1	SETBP1	SF3B1	SRSF2	STAG2
TET2	TP53	U2AF1	WT1	ZRSR2

Acute Myeloid Leukemia (AML) Panel (30 genes)

Test Code: TL94-7

ASXL1	BRAF	BCOR	CEBPA	CSFR3	DDX41	DNMT3A	ETV6	EZH2
FLT3	GATA2	HRAS	IDH1	IDH2	JAK2	KIT	KMT2A	KRAS
NF1	NPM1	NRAS	PDGFRA	PHF6	PTPN11	RUNX1	SETBP1	STAG2
TET2	TP53	WT1

Myeloproliferative Neoplasms (MPN) Panel (17 genes)

Test Code: TL96-2

ABL1	ASXL1	CALR	CBL	CSF3R	DNMT3A	EZH2	IDH1	IDH2
JAK2	KIT	MPL	SETBP1	SF3B1	SRSF2	TP53	U2AF1

MPN Cascade Sequencing Panel (3 genes)

Test Code: TL97-4

JAK2 (exon 12 and V617F)

CALR

MPL

Myelodysplastic Syndrome (MDS) Panel (18 genes)

Test Code: TL98-8

ASXL1	BCOR	CBL	DNMT3A	ETV6	EZH2	JAK2	KRAS	NRAS
PTPN11	RUNX1	SETBP1	SF3B1	SRSF2	TET2	TP53	U2AF1	ZRSR2

ONKOSIGHT ADVANCEDTM NEXT GENERATION SEQUENCING

Solid Tumor

Test Code TJ16-5

AKT1

APC

ARID1A

ATM

BRAF

BRCA1

BRCA2

CDH1

CDKN2A

CTNNB1

EGFR

EPCAM

ERBB2

ERBB4

FBXW7

FGFR1

FGFR2

FGFR3

GNA11

GNAQ

GNAS

HRAS

IDH1

IDH2

KDR

KIT

KRAS

MEN1

MET

MLH1

MSH2

MSH6

NOTCH1

NRAS

PDGFRA

PIK3CA

PMS2

POLE

PTEN

PTPN11

RB1

RET

SMAD4

SMO

STK11

TERT

TSC1

TSC2

TP53

VHL